http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-284898-A5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da9c519bc5d52cdf9e2c478e123d9f7b |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-70 |
| filingDate | 1989-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_158b20ee64500c5904b292e01d6d09fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d01ff565ba7241a1d693bca1a166fcba |
| publicationDate | 1990-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-284898-A5 |
| titleOfInvention | PROCESS FOR THE PRODUCTION OF AUTHENTIC SEROTYPE C STREPTOKINASE |
| abstract | The invention relates to a genetic-microbial method for obtaining authentic serotype C streptokinase. It aims to effectively produce the said protein. The task associated with this objective is achieved as follows: First, a cell population of one strain of the species Streptococcus equisimilis is transformed with a recombinant plasmid vector replicable in Gram-positive bacteria which, in addition to the coding DNA, encodes the coding DNA sequence for at least one phenotypic marker that can be expressed in Streptococcus authentic serotype C streptokinase carries. Transformed recipient cells obtained here are subsequently amplified for 8 to 20 generations without selection pressure with respect to the plasmid-encoded phenotype marker and checked for the combination of features plasmid-free and simultaneous preservation of said marker. As a result of this procedure, homologous recombination produces mutant S. equisimilis strains in which the skc gene is duplicated in the chromosome of the host. The novel S. equisimilis strains obtained in this way, such as the strain H46SM and H46SMS, are used for submerged fermentation, and from the culture fluid formed, the said protein is finally isolated in a manner known per se. {Authentic serotype C streptokinase; genetically-microbial process; Streptococcus equisimilis, recombinant plasmid vector; coding DNA; skc gene; Transformation; Propagation without selection pressure; Staemme H46SM and H46SMS} |
| priorityDate | 1989-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.