http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-283154-A5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da9c519bc5d52cdf9e2c478e123d9f7b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-18 |
| filingDate | 1989-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21762ffbccd9660be0d44cff178e9a18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37728a5f6225d75dd2ef3b36dec29f57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f4a677415bed60494cde91a97ab5899 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82c68970134aa583b25b300cb47fc3c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22dd9fcc5b34d03140855c4a89cc10b4 |
| publicationDate | 1990-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-283154-A5 |
| titleOfInvention | PROCESS FOR PREPARING MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA 1 |
| abstract | The invention relates to a method for obtaining monoclonal antibodies against human interferon-alpha 1 (HuIFN-alpha1), which are empowered for exclusive reaction against this. The aim of the invention is the production of monoclonal antibodies which are useful for immunaffinitaetschromatographic enrichment and the immunochemical determination of recombinant HuIFN-alpha1 (rHuIFN-alpha1). The method of the present invention is characterized by fusing spleen cells of mice immunized intraperitoneally and subcutaneously with rHuIFN-alpha1 with myeloma cells of the line FO in vitro, selecting the obtained hybridomas with specific antibody production, then performing single-cell cloning and making the resulting stable clones more common Way further propagated. By culturing the hybrid cell clones of lines H62 / 21B10 and H62 / 21B12, the new monoclonal antibodies ZIMET 21B10 and ZIMET 21B12 are obtained. The new hybridoma cell lines H62 / 21B10 and H62 / 21B12 were deposited under the numbers ZIM-0441 and ZIM-0442 respectively in the depository for cell lines in the Central Institute for Molecular Biology of the AdW of the GDR. Areas of application of the invention are human medicine, biology, veterinary medicine and biotechnology. {Production of monoclonal antibodies, specific reaction with interferon-alpha 1, intraperitoneal and subcutaneous immunization; Fusion with myeloma cells of the line FO; Selection; Einzelzellklonierung; Hybrid cell clones H62 / 21B10 and H62 / 21B12} |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8349331-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8557967-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7910707-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111087461-A |
| priorityDate | 1989-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.