http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-279266-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3bfedd8ba4800708cecfd357707f8f67 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 1989-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc06f102577cd23997df387651306018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a707ba9021bedffe4d4096e893c5d314 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43b4c934eda446d4c0eff22388239eed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_353f75df06891827f214157a4ff00078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84d111fdb2fe9aa9e6bc20ce95c94503 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bab4817ca2d3264f5ba2aa08516c528 |
| publicationDate | 1990-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-279266-A1 |
| titleOfInvention | PROCESS FOR THE PREPARATION OF MONOCLONAL ANTIBODIES TO HUMAN INTERFERON ALPHA-1 (HUIFN ALPHA-1) |
| abstract | The invention relates to a method for the production of monoclonal antibodies to various antigenic determinants of human interferon-alpha-1 (HuIFN-alpha-1). Areas of application are medical diagnostics, biotechnology and enzyme technology. The process according to the invention is characterized in that spleen cells of Balb / c mice immunized with pure HuIFN-alpha-1 are fused with Sp2 / 0 myeloma cells by means of polyethylene glycol and the resulting antibody-producing hybridomas are selected. Using a 2-site binding assay or an antibody-inhibiting assay, the epitope specificities of the various monoclonal antibodies (mAbs), products of the selected hybridomas, are analyzed. The thus characterized and selected hybridoma cell lines are used according to the invention for the production of monoclonal antibodies either in cell culture or by transplantation on mice (ascites formation). The obtained monoclonal hybridoma clones produce mAbs specific for HuIFN-alpha-1, each recognizing different antigenic determinants of HuIFN-alpha-1 and thus to construct test kits for the selective determination of HuIFN-alpha-1 and for immunoaffinity chromatography of recombinant or natural HuIFN-alpha-1 are suitable. The specific hybridomas were deposited at the Central Institute of Molecular Biology on September 29, 1988 under the following registration numbers: Registration No. Designation of the hybridoma cell-clone designation of the mABZIM-0396IV H2ZIM-IV produced by the hybridoma cell clones H2ZIM-0397IV D11ZIM-IV D11ZIM- 0399IV D5ZIM-IV D5 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8349331-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8557967-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7910707-B2 |
| priorityDate | 1989-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.