http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-276692-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3bfedd8ba4800708cecfd357707f8f67 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 1988-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d2b4e89fbcb21f7252e25b307d7bd00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1bdde53d8eb7b9e7e28bb0798c42d0d |
| publicationDate | 1990-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-276692-A1 |
| titleOfInvention | PROCESS FOR OBTAINING RECOMBINANT PROTEINS FROM GRAM-NEGATIVE MICRO-ORGANISMS |
| abstract | The invention relates to a method for obtaining recombinant proteins from Gram-negative microorganisms, which can be used in biotechnological production. The method for obtaining recombinant proteins from gram-negative microorganisms by lysing the microorganisms by ultrasound, centrifuging the cell lysate and isolating the recombinant protein from the pellet is characterized in that the lysis is carried out in neutral, detergent-containing phosphate buffer, the sedimented after centrifugation of the cell lysate Protein aggregates in strongly basic buffer containing denaturing substances, resuspended and then modified the readily accessible SH groups, then the remaining sedimented protein aggregates under drastic denaturing conditions, then adding SH- and / or NH-group modifying substances and the resulting protein mixture chromatographically cleans. In the process according to the invention, fewer process steps are necessary than in previously known processes and lower product losses can be observed. Since the desired product purity (see figure) is reached already after a chromathography run, the process is cheaper than the previously known ones due to lower reagent consumption. |
| priorityDate | 1988-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 160.