http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-270722-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3bfedd8ba4800708cecfd357707f8f67 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 |
| filingDate | 1988-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bc2a2e252c726b5e56c4f0db3b837e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_475529787eef94d3927dfdafdc80d073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6310833165199a5053483d12af4a1332 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81d1846331b728e5219822a229e0045f |
| publicationDate | 1989-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-270722-A1 |
| titleOfInvention | METHOD FOR PRODUCING CHEMICALLY MODIFIED PEROXIDASE |
| abstract | The invention relates to a process for the preparation of chemically modified peroxidase, which can be used in all cases of application of the peroxidase enzyme reaction. It can be used in the spectrophotometric or electrochemical analysis of H2O2 or of H2O2-producing or H2O2-consuming processes. Fields of application of the invention are clinical chemistry, immunology, food chemistry, enzymology and biotechnology, especially in biosensors. According to the invention, peroxidase is reacted with a sulfonic acid chloride of a compound having electron transfer promoting properties, e.g. As an aromatic dihydroxy derivative or a chelate compound with redox properties, reacted with or without the addition of a basic substance. The chemically modified peroxidase thus obtained has a four- to five-fold higher enzyme activity compared to the unmodified starting peroxidase. In addition, the peroxidase modification compares to the starting peroxidase to stabilize the peroxidase enzyme activity both in terms of a temporal extension of the peroxidase Reaktionsfaehigkeit as well as with respect to a temporally significantly extended storage possibility of the modified peroxidase in aqueous solution. When using disulfonic acid dichlorides of the compounds mentioned as modifying reagent and adding a carrier compound containing NH.sub.2 groups, the chemical modification of the peroxidase and the coupling of the chemically modified peroxidase to the carrier compound take place in one process step. |
| priorityDate | 1988-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.