Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af7c9c966acd2ef7eca105d82d8d0991 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2730-10122 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate |
1986-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
1988-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
DD-255544-A5 |
titleOfInvention |
Method for the site-selective gene modification of yeasts of the genus Pichia |
abstract |
A method for site-specific gene modification of yeasts of the genus Pichia and novel DNA sequences suitable for this purpose are described. Pichia is transformed with a serially arranged linear DNA fragment containing first and second insertable DNA fragments flanking a marker gene. The insertable DNA sequences are homologous to defined regions of the Pichia genome and are integrated by recombination at these defined sites. The resulting transformed organism contains the marker gene and any additional DNA sequences located between insertable DNA fragments. Further, the resulting transformed organism has a broken or deleted sequence at the site of the genome modification. Increased production of heterologous gene products is observed when the host is used for expression of stems in which the alcohol oxidase gene has been disrupted. After the disruption of the primary alcohol oxidase gene of Pichia, the existence of a second alcohol oxidase gene was discovered in Pichia. |
priorityDate |
1985-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |