http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-249283-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8bb618e58437830d578d482f70ddeb8d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 1986-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dc45ad42f7286f55d40a6c22d221217 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88109b263279d046970f3e8ab6f93329 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e27e5c05c17182f1e4c18cfaea610d66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ca1d05a4904936ff99768190071c9ac |
| publicationDate | 1987-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-249283-A1 |
| titleOfInvention | PROCESS FOR THE PREPARATION OF OLIGONUCLEOTIDES WITH RANDOMIZED SEQUENCE AND THEIR USE FOR THE GRAINED DNA SYNTHESIS |
| abstract | The method finds application in the preparation of DNA fragments from natural DNA and their use as primers in cDNA synthesis and oligo-labeling. The method allows the preparation of the primer DNA without elaborate fractionation techniques. The essence is that the DNA is digested sequentially with two endonucleases (DNase I and micrococcal nuclease), with DNase I treatment in Mn-containing solution. As a result, fragments are obtained which show virtually no self-installation of radioactivity during the synthesis reactions. Additional fractionation is achieved by differential ethanol precipitation. |
| priorityDate | 1986-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 151.