http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-248604-A1

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52
filingDate 1984-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35e6f1353cc98b692b5c2d9ab27fbac0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f44c037329f59ec51633e84b35257c36
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publicationDate 1987-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DD-248604-A1
titleOfInvention METHOD FOR PRODUCING NUCLEASE AND ANTBIOTIC-FREE PROTEOLYTIC ENZYME COMPLEXES
abstract The process for the preparation of a nuclease- and antibiotics-free proteolytic enzyme complex from crude enzyme complexes from Antibiotikafermentationen has the task to produce nuclease and antibiotic-free proteolytic enzyme complexes from known in a known manner Rohenzym complexes. The object is achieved according to the invention in that the crude proteolytic enzyme complex comprises a combination of special purification and treatment steps consisting of neutral salt precipitation in the presence of water-miscible solvents, alumina and activated carbon treatment in the presence of 5 to 15% water-miscible solvents and heat denaturation for 10 to 20 min at 65C in the presence of serine, asparagine and 1 to 5 mM CaCl2.
priorityDate 1984-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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