| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f68254c2256a29224bb518f200d45ce9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-814
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-816 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 |
| filingDate |
1984-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4d4710a8c50a9db02a4a8221eb1dac0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19f0df2dad35f50562fbd5a2db79418f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5e9f42eb57623da91b5d9bf81eaeb8e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a5210848f1faf18c1cfd7934285c6ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ecdb4b03df7acb6d64eb4617feeb769 |
| publicationDate |
1985-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
DD-220334-A5 |
| titleOfInvention |
METHOD OF ISOLATING THE CARBOXYPEPTIDASE B ENZYME FROM THE PANCREAS OF SAEUGETS |
| abstract |
HOMOGENIZED PANCREAS ARE AUTOMATED BY PUMPING THE AUTOLYSATE OBTAINED THROUGH A BUFFER OF 5.0-8.5 IN A 1: 1 CONVENTION AND SUBJECTING IT AT 50-75 DEGREES CELSIUS FOR 5-30 MINUTES OF A HEAT TREATMENT. The mixture thus obtained is cen- trifuged, and the supernatant solution is added 200-320 μg / ml of ammonium sulphate. THE SUSPENSION IS CENTRIFUGED, THE RECEIVED FLUID IS ACCOMPANIED TO 10-80 G / I AMMONIUM SULPHATE. THE RECEIVED ACTIVE CARBOXYPEPTIDASE ENZYM WELCOMES, THE PRECIPITATE IS CENTRIFUGED, THE RECEIVED ENZYME WATER WATER OR A BUFFER SOLUTION AND DIALYSIS AGAINST WATER OR A BUFFER SOLUTION. THE RECEIVED PRODUCT IS STORED IN FROZEN CONDITION. |
| priorityDate |
1983-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |