http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-204796-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da9c519bc5d52cdf9e2c478e123d9f7b |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
filingDate | 1982-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_894f84c79671a8160da04df6dd75b97c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_913647c82fcce3ba381c97433e2ce406 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61c76be198682df64cf9dc402ee5ee41 |
publicationDate | 1983-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DD-204796-A1 |
titleOfInvention | PROCESS FOR THE PREPARATION OF VECTOR PLASMIDES FOR E. COLI |
abstract | The invention relates to a method for obtaining new vector plasmids for E. coli and pursues the goal of providing biochemical tools for genetic engineering and molecular biology research. The task of describing a suitable method is solved in such a way that by means of in vitro recombination of the plasmid pBR322 with DNA of Streptomyces phage SH10 bzw.mit isolated fragments of SH10-DNA, by transformation of E. coli HB101, using Selection and propagation of the recombinant clones and subsequent isolation by density gradient centrifugation new vector plasmids can be obtained. In view of the hereby introduced unic cleft sites for Kpn I and Bgl II, these plasmids represent a new quality among the vectors for E. coli. |
priorityDate | 1982-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.