| abstract |
Described are methods and means for the construction and microbial expression of quasi-synthetic genes resulting from the combination of organic synthesis and enzymatic reverse transcription of messenger RNA sequences that are incomplete from the point of view of the desired protein product. Preferred expression products lack bioinactivating leader sequences common in eukaryotic expression products, but problematic in terms of microbial cutting which provides the bioactive material first. A preferred embodiment is more specifically constructed and expressed in which a gene coding for human growth hormone (which is required, for example, for the treatment of pituitary dwarfism) is constructed and expressed. |