http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-140476-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46896e96d197d20b14ed77cb7f86a3bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_16e6ca41c4658a36a5fb141cd5a61547 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_87bc75f3ba63c0003f2c711782b989f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c134600c41f0c170c050b34a95d7411 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d351a7d4c17072f400e466833b182f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3ed8661d6a1e61c02302dddc93486d42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 1978-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6aa2024dfa4974387bc4a3f5186f7be0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f939ea85ba95ffa625ab135502b0188c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ac0f8a0788b07c6042e25f62b2a7717 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7173175e33cc976f6656e60ebd6f05ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b2b79fd5c9247b57845b3b15c73d7d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0894859bb55e5cb1f0396a8ef1bf44b |
| publicationDate | 1980-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-140476-A1 |
| titleOfInvention | METHOD FOR INCREASING THE STABILITY OF TEMPERATURE OF CONSERVATED LIVING CELLS |
| abstract | The invention relates to a method for increasing the stability of cells to freeze / thaw damage in cryopreservation. It can be used anywhere where living cells, especially for the purpose of retransmission to similar organisms, should be kept for a long time and kept in stock. The aim of the invention is to significantly increase the membrane stability of the cells and thus their survival outside the organism in comparison to previous possibilities. It is the object of this to develop a suitable method using a stability-increasing active ingredient or a combination of active ingredients. The method according to the invention is characterized in that the preservation solution contains seleno-amino acids, e.g. Seleno-methionine, or a combination of sodium selenite and tocopherol (vitamin E) in a concentration of 1.0 to 10.0 Myg / 100 ml, then the cells to be preserved are suspended therein and incubated for a maximum of 3 h to 37 degrees C. , subsequently freezing in a cryoprotective medium while maintaining a suitable cooling rate and storing in liquid nitrogen. With this method, a high membrane stability of the cells is achieved compared to previously applicable preservation techniques. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0394788-A1 |
| priorityDate | 1978-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.