| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fdaa64756184e715af6cb25ebc318917 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-77
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-465 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-465
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-281
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate |
1996-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_235498c5a05834bc3f7fb665b2642ec2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a542bc59f0b3197c5032240ada5eb3a0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c3562c92f11363dcdf93efbd6fab7b9 |
| publicationDate |
1998-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CZ-401697-A3 |
| titleOfInvention |
Modified biotin-binding avidin-type molecule, use thereof, nitro-tyrosine natural avidin and method for its production, bacterial streptavidin, biotinylated ligand immobilization column, and method for obtaining avidin column or biotinylated ligand |
| abstract |
Biotin-binding modified avidin-type molecules are provided in which the essential tyrosine residue in the bioting-binding site is modified in such a way that its pKa is decreased in comparison to the pKa of the unmodified tyrosine residue in the corresponding unmodified avidin-type molecule. The avidin-type molecules include: (i) native egg-white avidin; (ii) recombinant avidin; (iii) deglycosylated forms of avidin; (iv) bacterial streptavidin; (v) recombinant streptavidin; (vi) truncated streptavidin; and (vii) derivatives of (i)-(vi) which are modified at sites other than the essential tyrosine residue. The modification is achieved by substitution at one or both ortho positions to the hydroxy radical of the tyrosine residue by radicals such as nitro, halogen, azo and amino. The modified avidin-type molecules can be used in all applications of the avidin-biotin technology. |
| priorityDate |
1995-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |