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filingDate 2016-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CZ-307807-B6
titleOfInvention A method for increasing the signal of antibodies recognizing 5-bromo-2'-deoxyuridine and / or 5-chloro-2'-deoxyuridine and / or 5-iodo-2'-deoxyuridine at the sites of incorporation of these nucleosides into DNA after DNase I treatment
abstract The method allows to efficiently increase the signal of antibodies recognizing 5-bromo-2'-deoxyuridine and / or 5-chloro-2'-deoxyuridine and / or 5-iodo-2'-deoxyuridine in DNA at sites of incorporation of these nucleosides into DNA after DNase I The method consists in incubating samples, after treatment with DNase I and antibody, in formaldehyde solution and is useful both when two antibody-based and antibody-based systems are conjugated to different fluorochrome or biotin-type substances.
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