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publicationDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CZ-304856-B6
titleOfInvention A method for modifying an endogenous or chromosomal locus
abstract A method for modifying an endogenous gene or a selected chromosomal locus in a non-human eukaryotic cell is described, comprising using bacterial homologous recombination to modify a large cloned genomic fragment larger than 20 kb to create a large specifically targeted vector (LTVEC); use of LTVEC to modify an endogenous gene or chromosomal locus with a large cloned genomic fragment to create a modified allele; and detecting the modified allele to modify the endogenous gene or selected chromosomal locus in the non-human eukaryotic cell.
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