http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-304351-B6
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5bf2747f075a30d3e4541f0b76404e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36e8afc6e43a907b0d9bf79ca7205d87 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-35 |
| filingDate | 2012-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ea01672410f7458191c133cd3f54119 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cd58deb5df02b06c99194283891a229 |
| publicationDate | 2014-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CZ-304351-B6 |
| titleOfInvention | Method of preparation of excrement antigens of domestic mites |
| abstract | The present invention relates to a process for the preparation of excrement antigens of domestic mites, which includes both the steps of the mite breeding and the steps of extracting the antigens, where excrements adhered to the surface of the breeding vessel during breeding serve as a source of pure excrement of domestic mites of at least 95% by volume. Only 1 to 5 mg of growth medium per 1 cm 2 is used during mite breeding. the breeding area, the growth medium never forms a continuous layer and is added sequentially. The mite rearing has been running for a maximum of 8 weeks since its establishment, with the rotation of breeding containers with mite cultures during breeding at regular time intervals to ensure that the excrement is uniformly adhered to all walls of the breeding vessel. After covering the breeding containers with mite excrements in the continuous layer, the residual growth medium which does not serve as a source of mite excrement antigens is removed and subsequently, after previous cold paralysis, live mites are removed on the adhering walls, thereby obtaining (i) 95% vol. adhering to the walls of the breeding containers; (ii) feeding on a mite of 95% vol minimum purity; and (iii) residual growth medium containing less than 50% by volume live mites. Extraction of the antigens from the mite excrements adhered to the walls of the breeding vessel with a minimum purity of 95% by volume is carried out with water shaking directly in the breeding vessel after the mite breeding is finished, thereby directly extracting the excrement protein extract from which the supernatant is obtained after centrifugation. Finging the supernatant without any further purification steps to validate the composition and integrity of the prepared mite antigens from mite excrements. |
| priorityDate | 2012-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.