http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-303251-B6
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8faf71a78bf0e9b2d4b7357812d84ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_babd1da3f5bb8fb4167435b1a79d3a90 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2011-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f5da5fc6fc91c74d31b0c68a7bf8e65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d0374d65248f6143c6bb66413b9636b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_530cce5ab2ee1bc3e781551a1a393a5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93f20f004969eeebb7cea07f2afc408e |
| publicationDate | 2012-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CZ-303251-B6 |
| titleOfInvention | A method of labeling two-stranded DNA molecules |
| abstract | The method described allows two-stranded DNA to be effectively labeled. The method consists in incubating double-stranded DNA in the presence of monovalent copper and oxygen ions and / or active oxidants. The source of these ions may be aqueous solutions containing copper ions, or may be a monovalent honey that has been bound to the DNA. Due to the action of oxygen and monovalent copper ions, DNA strands result in breaks that serve as the starting point for DNA strand synthesis using these enzymes in the subsequent step of incubation with labeled nucleotides and enzymes with DNA polymerase activity. Either terminal deoxynucleotidyl transferase, or DNA polymerase, synthesizing the novel chain according to the master strand may be used. If these DNA polymerases do not have exonuclease activity and / or displacement activity, it is necessary to incubate samples containing double stranded DNA with enzymes having exonuclease and / or phosphatase activity prior to incubation with DNA polymerase to ensure sufficient signal strength. The incubation step with these enzymes can also be used to increase the signal when subsequent DNA polymerases with exonuclease and / or displacement activity are used. In the case of terminal deoxynucleotidyltransferase, it is advantageous to incubate in the presence of an enzyme with 3'-5 'exonuclease and / or phosphatase activity prior to incubation with this enzyme. Rinsing agents may optionally be used to remove unwanted copper products. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-307016-B6 |
| priorityDate | 2011-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 300.