http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-302216-B6
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e43ee7489a10533b15d9c576669c8d5 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-66 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-60 |
filingDate | 2009-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71a01c28c3997390e690f9340b1f42b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae49e161f6c186caa2fec7b1515b9e68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fc42bb93edbddbd17d830fd8e2ed7ec |
publicationDate | 2010-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CZ-302216-B6 |
titleOfInvention | A method for producing quercetin-3-beta-D-glucopyranoside to form L-rhamnose |
abstract | A method for producing quercetin-3-beta-D-glucopyranoside (isoquercitrin) by enzymatic cleavage of rutin to form L-rhamnose by-product. The solution or suspension of rutin in aqueous solution, at a concentration of 11 to 350 g / l, is caused by an enzyme selected from the group of glycosidases including .alpha.-L-rhamnosidase and naringinase, at temperatures of 60-90 degC and at pH v. ranging from 8.05 to 10.1. Upon completion of the reaction, the isoquercitrin is separated, optionally purified by recrystallization from a strongly basic solution, e.g. sodium hydroxide solution. After separation of the isoquercitrin, the remaining L-rhamnose-containing reaction solution can be further used to prepare the alpha-L-rhamnosidase enzyme by submerged cultivation of a Aspergillus or Penicillium microorganism and preferably Aspegillus terreus in an L-rhamnose-inducing medium. In addition, the remaining reaction solution containing still active glycosidase, after dilution and pH adjustment, can be reused directly to further convert rutin to isoquercitrin. The glycosidase used is preferably alpha-L-rhamnosidase, both commercially available and submerged. |
priorityDate | 2009-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.