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filingDate 2009-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71a01c28c3997390e690f9340b1f42b3
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publicationDate 2010-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CZ-302216-B6
titleOfInvention A method for producing quercetin-3-beta-D-glucopyranoside to form L-rhamnose
abstract A method for producing quercetin-3-beta-D-glucopyranoside (isoquercitrin) by enzymatic cleavage of rutin to form L-rhamnose by-product. The solution or suspension of rutin in aqueous solution, at a concentration of 11 to 350 g / l, is caused by an enzyme selected from the group of glycosidases including .alpha.-L-rhamnosidase and naringinase, at temperatures of 60-90 degC and at pH v. ranging from 8.05 to 10.1. Upon completion of the reaction, the isoquercitrin is separated, optionally purified by recrystallization from a strongly basic solution, e.g. sodium hydroxide solution. After separation of the isoquercitrin, the remaining L-rhamnose-containing reaction solution can be further used to prepare the alpha-L-rhamnosidase enzyme by submerged cultivation of a Aspergillus or Penicillium microorganism and preferably Aspegillus terreus in an L-rhamnose-inducing medium. In addition, the remaining reaction solution containing still active glycosidase, after dilution and pH adjustment, can be reused directly to further convert rutin to isoquercitrin. The glycosidase used is preferably alpha-L-rhamnosidase, both commercially available and submerged.
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