| abstract |
The invention relates to a human DNase I variant having DNA hydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence of natural human DNase I shown in SEQ. ID.C. 1, and at least one amino acid substitution at a position selected from the group consisting of H44Q, L45K, L45R, V48K, V48R, G49R, G49Y, G49I, G49K, L52K, L52M, L52R, D53L, D53M, N56F, N56K, N56R, N56W, Y65K, Y65M, Y65P, Y65S, V66N, V67D, V67M, V67M, V67P, V67R, V67S, S68K, S68M, S68R, E69M, A114E, A114M, A114M, A114Q, A114R, A114W, A114Y, S68N: P70T, S94N: Y96T, V67N: E69T, Y65N: V67T, V66N: S68T, S68N: P70S and S94N: Y96S. |