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publicationDate 2005-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CZ-294950-B6
titleOfInvention A method of cryopreserving mammalian tissues and cultured tissue equivalents
abstract The cryopreservation method of the obtained mammalian tissue or cultured tissue equivalent prepared in vitro is performed when mammalian tissue is mammalian skin and the cultured tissue equivalent is the equivalent selected from the group consisting of cultured epidermal equivalent, cultured dermal equivalent, and cultured corneal equivalent. The tissue or cultured tissue equivalent is immersed in a cryogenic preservative solution and said cryogenic preservative solution and submerged tissue are mixed to achieve effective tissue penetration and tissue perfusion, and then the tissue freezes at a very low rate of -0.3. degree.C or lower. During freezing, extracellular ice formation is induced by nucleation. Cryopreserved tissue can be stored indefinitely prior to use
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