| abstract |
There is described an expression vector providing extracellular production of Apo AI-M (Milano) using Escherichia coli, which includes a plasmid carrying the replication sequence, an inducible promoter sequence selected from the lac promoter, trp promoter, hybrids between lac the promoter and the trp promoter, and their derivatives, the DNA sequence for the signal peptide, the DNA sequence for the apo AI-M apolipoprotein and the transcript terminator. The Escherichia coli strain, which is transformed with the above expression vector, is described below. |