| abstract |
It is described for an isolated nucleic acid comprising a nucleotide sequence that provides a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 2 and which has the enzymatic activity of the stratum corneum chymotryptic enzyme, the replicable expression vector pS507 DSM 8282, as well as the cell or cell line that carries this vector, and plasmid pS500 DSM 8281. Thus, essentially a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 2, as well as the method of preparation, is described. (b) transforming the appropriate host organism with the vector thus scanned, (c) culturing the organism under conditions suitable for expression of the polypeptide; and (e) the pop pad is subjected to a post-transla n polypeptide modification. The pharmaceutical precautions for the use of disease contains the polypeptide. |