abstract |
The cytotoxic T cell, its TcR contains heterologous alpha. and .beta. polypeptides, wherein these heterologous polypeptides provide the lymphocyte with limited MHC specificity for cells that cause disease, the T lymphocyte being monovalent and one type of TcR, providing limited MHC specificity of the Even for one cell number, the production of cytotoxic T-lymphocytes is due to the fact that a vector is formed, containing DNA, preferably DNA derived from the cytotoxic T-lymphocyte, to the duj c .alfa. and .beta. TcR polypeptides specific for disease-causing cells, (b) transfect the recipient cytotoxic T-lymphocyte with the vector from the scans in step (a) to produce recombinant cytotoxic T-lymphocyte that DNA for the cuja .alfa. and .beta. Cell-specific TcR polypeptides causing disease, recombinant cytotoxic T-lymphocyte from step (b), express DNA to alpha. and .beta. TcR polypeptides provide limited MHC class I specificity to the lymphocytes, and are targeted to cells. The vector for use in the method includes c DNA for alpha. and .beta. Disease-specific TcR polypeptides, including DNA operably linked to an expression element or expression elements, express the expression element or expression elements, for example, from transcript and / or translase elements, promoters, ribosomes, helper elements, regulatory sites, with activator and repressor sites and expression elements naturally associated with .alpha. and / or .beta. TcR peptide genes. |