| abstract |
The present invention relates to proteinase K variants having a modified amino acid sequence of wild-type proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type proteinase K. The present invention also relates to DNA sequences encoding such proteinase K variants. The present invention also relates to compositions comprising such proteinase K variants for cleaning a variety of surfaces. |