http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-2014579-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90b77cef6ab2c0daf477355fe7ede9f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d2393da59d45c028cec2fa2ddc3011d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-978 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2014-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c80ff9029f4569aff7d36f32eb00d690 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_447e9340280df00b57613091e896fa24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b22fd9a2775b0f02ce75d5edc5471c60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fce46a17e5d35a2337fbdcea2bd78240 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af9e7f8751390413991c2d8bc4a45ba8 |
| publicationDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CZ-2014579-A3 |
| titleOfInvention | A method for determining the activity of enzymes converting cytosine derivatives into uracil derivatives in cells, tissues, and organisms |
| abstract | The present invention provides a method for determining the deaminase activity of cytosine derivatives converting enzymes to uracil derivatives in a sample by incubating the sample with an enzyme substrate selected from the group consisting of 5-ethynyl-2'-deoxycytidine (EdC), 5-bromo-2'-deoxycytidine (BrdC) , 5-chloro-2'-deoxycytidine (CldC), 5-iodo-2'-deoxycytidine (IdC), 5-ethynylcytidine (EC), 5-bromo-cytidine (BrC), 5-chloro-cytidine (ClC), 5-iodo-cytidine ( IC) and 5-fluorocytidine (FC), these substrates being converted to deaminated products in the presence of cytosine derivatives to uracil derivatives, EdC is converted to 5-ethynyl-2'-deoxyuridine (EdU), BrdC to 5 -bromo-2'-deoxyuridine (BrdU), CldC to 5-chloro-2'-deoxyuridine (CldU), IdC to 5-iodo-2'-deoxyuridine (IdU), EC to 5-ethynyluridine (EU), BrC to 5-bromuridine (BrU), ClC to 5-chloruridine (ClU), IC to 5-ioduridine (IU) and FC to 5-floururidine (FU), these deaminated products are subsequently detected in DNA or R NA and determined in the amount of incorporated EdU, BrdU, CldU, respectively. IdU in DNA and EU, BrU, ClU, IU, resp. FU in RNA. The present invention also includes the use of the aforementioned method for determining deaminase activity in cells treated with substances that affect the deaminase activity of cytosine derivative converting enzymes to uracil derivatives. |
| priorityDate | 2014-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 249.