| abstract |
A cross-linked derivative of hyaluronan in the form of a hydrogel or microfibre and a method for its preparation, comprising a C-C coupling reaction. The C-C bonding reaction is carried out by reacting a hyaluronan derivative bearing a terminal aryl halide and / or aryl borate group, and a hyaluronan derivative carrying an adenyl or alkynyl group, in water, a phosphate buffer or a mixture of organic acid and alcohol, and in the presence of a palladium active catalyst. The palladium active catalyst may be, for example, a palladium acetate complex and an inorganic or organic base, or a complex of Pd (II) and 2-amino-4,6-dihydroxypyrimidine. |