| abstract |
Assay method consisting of hydrolysisnurea immobilized urease and colorimetricndetermination of the released ammonia flownby injection analysis, epocive in what a donthe phosphate buffer carrier stream is appliedncontaining urea, carrier current ss uvsdencontact with immobilized urease locatednsolid on the support in the enzyme reactor and afternthe passage of DC with a stream of ammonia releasednsequentially mixed with reagent 1 containing itnIn 0.1 to 0.5 M sodium salcylan, 1 to 8 mMnsodium borohydride, o, 2 to 3 M hydroxiden% of alkali metal and up to 4% by volume of methanol,ng and with reagent II containing o, ol to 0.5 M chloroformnalkali metal, whereupon it warms upnη »the temperature of 29 to 60 ° C and determined photometricallynabsorbance at 7oo yum from which to determinenthe amount of urea in the sample to be analyzed |