abstract |
The invention relates to aerobic cell culturenEscherichia coli for preparationn6-aminopeniclanic acid by cleavagenbenpylpenicillin. Cultivation is performednin a liquid nutrient medium having a pH of 7.5 ton8.2, in the presence of sucrose, inorganicnsalts and buffer substances. After exhaustionnthe main source and the \ tncarbon is added in several portionsnphenylacetic acid and its ammonium salt, in totaln2.0% vol. |