| abstract |
The present invention relates to a method of selection and accumulationnmicroorganismsnphenoxymethylpenicillin 6-aminopeniailic acidnand It is marked by being culturenmicroorganisms are inoculated into selectivenmedia, where as the sole source of assimilationnnitrogen, and / or both nitrogen and nitrogenncarbon serves phenoxyacetamide, 3-phenylpropionamide,n4-hydroxyphenoxyacetamide, phenylacrylamide,nand / or, where as the sole sourcesnassimilable carbon serves the acid saltsn3-phenylpropionic, phenylacrylic, phenoxyaceticnand 4-hydroxyphenoxyacetic acid, optionally snthe only amides of these acidsnsources of assimilable nitrogen, or with inorganicnnitrogen source, preferably ammoniumnions, with grown culturesnmicroorganisms in these soilsnuntil the growth rate increases,nwhereupon the grown culture is vaccinated andnpreserves.nThe invention also relates to a strain of a microorganismnCryptococcus sp., CCY 17-22-1 producingnintracellular V-penicillin amidase,nwhich preferentially hydrolyses phenoxymethylpenicillinnto 6-aminopenicillanic acid. |