http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CO-6300794-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fba5ef972720f17c5f58279c41bebdf5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b701a17710d507e9ac4f0f43a02783d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5293b6635c40f8b5d5dd8921a3f442a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28a4af3570fb5fa2b28c3b2bf9023a2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ffe9daa3ac9a2f6fd16aa64b5f15648 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06cf77d1341f169d354b6ed8388efde6 |
| publicationDate | 2011-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CO-6300794-A2 |
| titleOfInvention | TRANSGEN MAMMALS THAT PRODUCE PROTEIN EXOGENA IN MILK |
| abstract | The invention relates to non-human transgenic mammals that are useful in the production of a protein of interest that can be toxic to mammals. The mammal is characterized by the fact that it is transgenic for the production in its milk of an inactive form of the protein of interest, preferably recombinant human insulin. It is not possible to produce recombinant human insulin in transgenic mammals because this molecule has a certain degree of biological activity in mammals and could be toxic to the mammal. Thus, the invention involves donating a genetic construct comprising a sequence encoding a modified human insulin precursor under the control of a beta casein promoter in an expression vector. This also involves transfecting the expression plasmid in fetal bovine somatic cells, such as fibroblasts, and enucleating bovine oocytes by nuclear transfer to generate transgenic embryos. The invention gives rise to a transgenic bovine that will be able to produce a modified precursor of human insulin in its mammary glands. Subsequently, the milk of these transgenic mammals can be collected, the modified human insulin precursor can be converted in vitro into recombinant human insulin, and the recombinant human insulin can be purified to homogenize it as a pure biopharmaceutical product. |
| priorityDate | 2007-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.