http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CO-6040047-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65800387fdad77475ba4a14258b0525d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-10 |
| filingDate | 2008-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_053ed91ead842281cbab3ef742d53178 |
| publicationDate | 2009-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CO-6040047-A1 |
| titleOfInvention | SARCOCYSTIDAE FAMILY PARASITES CULTURE |
| abstract | Cultivation of parasites of the Sarcocystidae family by means of a procedure comprising the steps of: - preparing a sporozoite solution as a culture base. - Prepare a suspension of bradozoites as a culture base. - prepare a culture medium Sarcocystidae - verify the existence of the toxin. Cultivation of parasites according to the method of claim 1 characterized in that the preparation of the sporozoite solution comprises the following steps: - dilute 1 g of bovine fecal matter in 20 cm of supersaturated saline solution whose density is between 1,200 g / cm and 1,400 g / cm- filter the infiltrate content of gauze, nylon or copper and centrifuge it - stand the centrifuge for 20-30 minutes - collect the supernatant zoites and wash them in a physiological solution of 0.9% NaCl and 0.7% formaldehyde - centrifuge 500 g for 15 minutes - perform 2 to 3 new washes with buffered physiological saline solution with a pH of 7.2 without formaldehyde - check the microscope concentration and purity of the sporozoites. Cultivation of parasites according to the method of claim 1 and characterized in that the preparation of the bradozoites comprises the steps of. - take a portion of cardiac or skeletal striated bovine muscle - clean it of fascia, nerves, tendons, fat and all non-striated tissue - shred it into 2 mm particles - pack it and cover it in a glass jar - add pancreatic trypsin solution because of : two parts of tripcin per one part of muscle - incubate for one hour at 37 ° C - filter in copper or nylon gauze sieve - centrifuge in filtered content in 500 g for 15 minutes - discard the supernatant and wash the sediment 3 times - Check the microscope for bradozoites and metrozoites of the parasite and its purity. <EMI FILE = "08122642_3" ID = "3" MFI = JPEG HE = 50 WI = 50> |
| priorityDate | 2008-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.