http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CO-5590971-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42e94091a4fc81b8d0237117707cc7ee |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70521 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2005-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_396c12fe0334f310086a90366e44e83b |
| publicationDate | 2005-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CO-5590971-A2 |
| titleOfInvention | IMPROVEMENT IN PRODUCT QUALITY IN CROP PROCESSES IN MAMMER CELLS FOR PROTEIN PRODUCTION |
| abstract | 1. The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cells or mammalian cell cultures, illustratively, but not limited to, fed batch cell cultures. The methods comprise feeding the cells with D-galactose, preferably with a feeding medium containing D-galactose, preferably daily, to sustain an effective sialylation level of D-galactose in the culture for its duration, thereby increasing sialylation. of the proteins produced. The methods may also comprise at least two temperature changes made during the culture period, in which the temperature is lower at the end of the culture period than at the time of starting cell culture. The cell culture process of the invention involves two or more temperature changes while maintaining high cell viability, and may allow an extended protein production phase. The methods may also comprise the delayed addition of the polyanionic compound at a time after inoculation. The complementation of the cultures with D-galactose, preferably in a feeding medium, to sustain the galactose at effective levels of sialylation in the cultures until the end of the culture run reversing a decline in the sialylation that accompanies the culture scale, and it is advantageous for large-scale cultivation processes. |
| priorityDate | 2002-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419588234 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID3037556 |
Total number of triples: 22.