| abstract |
Aspects of the present disclosure relate to a method of regenerating a bulk-loaded hydrophobic interaction chromatography column, the method comprising passing one or more column volumes of an alkaline solution through a means of hydrophobic interaction within the column, where the alkaline solution has a pH between about 10 and about 14, and a conductivity between 0.5 mS/cm and about 10 mS/cm, where material bound to the media is removed hydrophobic interaction. In some cases, the alkaline solution may include sodium hydroxide at a concentration of, for example, about 0.1 mM to 10 mM. |