| abstract |
ABSTRACT As a technique for proliferating neural crest cells without reducing the ability to differentiate, a method for producing neural crest cells is provided, comprising the steps of: (1) obtaining neural crest cells; and (2) suspending neural crest cells in a medium comprising a GSK3α inhibitor and a basic fibroblast growth factor (bFGF), wherein the medium comprises the GSK3α inhibitor with a concentration exhibiting an effect equivalent to that exhibited by CHIR99021 with a concentration greater than 1 µM and less than 5 µM. |