| abstract |
The utility model discloses a multistage sorting microfluidic device for rare cells. The multistage sorting microfluidic device comprises a runner structure and an electrode substrate which are encapsulated in a bonding manner, wherein the runner structure comprises a spiral runner, a secondary rubber, a lower branch runner, an upper bifurcated runner and a lower bifurcated runner, wherein a sample inlet is formed in one end of the spiral runner; the other end of the spiral runner is respectively connected with the secondary runner and the lower branch runner by a shrinking-expanding structure; the secondary runner is respectively connected with the upper bifurcated runner and the lower bifurcated runner; the upper bifurcated runner and the lower bifurcated runner are connected with a blood cell outlet; the lower bifurcated runner is connected with a rare cell outlet; the electrode substrate comprises a substrate body, and two electrode groups which are arranged on the bottom of the secondary runner. The multistage sorting microfluidic device disclosed by the utility model is low in cost, simple to operate, and easy for micromation integration, and the high-flux high-purity separation of the rare cancer cells can be realized. |