| abstract |
The present invention provides a method of spatially separating at least a first and a second component of a sample, in an exemplary embodiment, the method comprising dissolving a first component of a carrier liquid containing a spacer electrolyte solution. and the second component are introduced into the first microfluidic channel of the microfluidic device, and then the first and second components are stacked between the leading electrolyte solution and the trailing electrolyte solution by isotachophoresis, wherein the interval electrolyte solution contains The electrophoretic mobility of the ions in the electric field is between the mobility of the ions present in the leading electrolyte and the trailing electrolyte, and the spacer electrolyte solution contains at least one of the following spacer ions: MOPS, MES, nonanoic acid, D- Glucuronic acid, acetylsalicylic acid, 4-ethoxybenzoic acid, glutaric acid, 3-phenylpropionic acid, phenoxyacetic acid, cysteine, hippuric acid, p-hydroxyphenylacetic acid, isopropyl Malonic acid, itaconic acid, citraconic acid, 3,5-dimethylbenzoic acid, 2,3-dimethylbenzoic acid, p-hydroxycinnamic acid and 5-bromo-2,4-dihydroxybenzoic acid, The first component comprises a DNA-antibody conjugate, and the second component comprises a complex of the DNA-antibody conjugate and an analyte. |