| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca32fd2a44fd326ffd027a75a57bd13f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1055
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-32 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate |
1997-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b3906239d918d618e50d83561d5fe6b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d0c43cdc7bebb3ddbe1bf5e327bd77b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f3c7d012e827b81f3c809f843d35283 |
| publicationDate |
1998-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-1200404-A |
| titleOfInvention |
Method of selection of Allelic exchange mutants |
| abstract |
A process for replacing a nucleotide sequence in the genome of a mycobacterium strain comprises the steps of: a) providing a vector containing SacB gene coding for levane saccharase enzyme and a nucleotide sequence of interest; b) transfecting the mycobacterium strain with the vector; c) selecting clones of the resulting transfected mycobacteria for replacement of the nucleotide sequence of interest by propagating the transfected clones in a culture medium supplemented with sucrose; and d) isolating the recombinant strain. The process is useful for positive selection of allelic exchange mutants, such as in Mycobacterium tuberculosis complex. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951627-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951627-B |
| priorityDate |
1996-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |