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filingDate 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57bf8ca26bd3eeba7df11e136e6cd2e1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9eb0bedbd67a2410e47c7b3e3c642243
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publicationDate 2022-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115247208-A
titleOfInvention A real-time fluorescence quantitative qRT-PCR detection method and kit for IL-1β
abstract The invention discloses a real-time fluorescence quantitative qRT-PCR detection method and kit for IL-1β. The real-time fluorescence quantitative qRT-PCR detection kit for IL-1β provided by the present invention includes a primer-probe combination consisting of an upstream primer, a downstream primer and a probe for detecting the expression level of the IL-1β gene, and the upstream primer is the SEQ ID in the sequence table. The single-stranded DNA shown in ID No.1, the downstream primer is the single-stranded DNA shown in SEQ ID No.2 in the sequence listing, and the probe passes through the 5' of the single-stranded DNA shown in SEQ ID No.3 in the sequence listing. The end-labeled fluorescent group and the 3' end-labeled fluorescent quenching group are obtained. The kit of the invention can realize the rapid and accurate detection of IL-1β gene expression with high sensitivity, high specificity and high throughput, can be widely used in the detection of IL-1β in clinical and laboratory, and can be widely used in clinical and laboratory.
priorityDate 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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