http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115247208-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7cc315cc5a1fe3bf4c1730dd5a48c1f2 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
filingDate | 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57bf8ca26bd3eeba7df11e136e6cd2e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9eb0bedbd67a2410e47c7b3e3c642243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_496f1a4a04a97ef2efaab298feb0bf16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9813880356618b54cf348738f2522b31 |
publicationDate | 2022-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-115247208-A |
titleOfInvention | A real-time fluorescence quantitative qRT-PCR detection method and kit for IL-1β |
abstract | The invention discloses a real-time fluorescence quantitative qRT-PCR detection method and kit for IL-1β. The real-time fluorescence quantitative qRT-PCR detection kit for IL-1β provided by the present invention includes a primer-probe combination consisting of an upstream primer, a downstream primer and a probe for detecting the expression level of the IL-1β gene, and the upstream primer is the SEQ ID in the sequence table. The single-stranded DNA shown in ID No.1, the downstream primer is the single-stranded DNA shown in SEQ ID No.2 in the sequence listing, and the probe passes through the 5' of the single-stranded DNA shown in SEQ ID No.3 in the sequence listing. The end-labeled fluorescent group and the 3' end-labeled fluorescent quenching group are obtained. The kit of the invention can realize the rapid and accurate detection of IL-1β gene expression with high sensitivity, high specificity and high throughput, can be widely used in the detection of IL-1β in clinical and laboratory, and can be widely used in clinical and laboratory. |
priorityDate | 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.