| abstract |
The method discloses a conotoxin mutant, the amino acid sequence of the mutant is SEQ ID NO: 1GCCX 1 X 2 X 3 ACX 4 X 5 X 6 X 7 X 8 X 9 X 10 CX 11 ; wherein, X 1 is selected from S, T, A, R, Alg, Dap or Dab; X 2 is selected from H, R, W, A or N; X 3 is selected from P or Hyp; X 4 is selected from S, L, V, A , D, K, T, Dab, Dap or Y; X 5 is selected from V, L, A, Y, R, Cit, Dap, Dab, D or K; X 6 is selected from N, Q or A; X 7 is selected from X 8 is P or A; X 9 is selected from D, E, S or A; X 10 is I or A; X 11 is C-terminal amidation, or, selected from Y, L or R . The present invention also provides preparation methods and applications. The conotoxin mutant provided by the present invention has obvious analgesic effect and is not addictive. |