abstract |
The invention discloses a method for screening the effective substances of microorganisms for regulating rumen function, which comprises the following steps: collecting animal rumen juice, adding substrate ration and yeast culture, and culturing in a closed and constant temperature; Effect index; carry out correlation analysis, screen out the metabolites (x) that are positively correlated, and establish a single regression equation y=f( xi ) with x and MFAEI (y); the x is the effective substance for microorganisms to regulate rumen function ; rumen function regulating effective composition, it is composed of propionic acid, oxalic acid, lyxose, 5-hydroxytryptophan, 3-a-mannan, turanose; the advantage is: the establishment of yeast culture Mathematical statistical relationship between each metabolite in and the rumen fermentation comprehensive index, identified the efficacy substances in yeast culture to promote rumen fermentation; estimated the quantitative relationship between several efficacy substances and the efficacy substances in yeast culture ideal level. |