http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115236328-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57484 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6839 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-561 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2022-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115236328-B |
| titleOfInvention | A detection method and application of human cell protein deamidation based on two-dimensional electrophoresis |
| abstract | The invention discloses a two-dimensional electrophoresis-based detection method for deamidation of human cell proteins and its application. The method includes: S1, cell culture and treatment: adding 6-diazo-5 to primary cultured human cells ‑Oxo‑L‑norleucine treatment or the samples obtained by introducing lentivirus infection into shRNA as the experimental group, and the corresponding blank control group, and collecting cell samples after culture; S2, making cell samples into two-dimensional electrophoresis Sample; S3, two-dimensional electrophoresis; S4, SDS-PAGE polyacrylamide gel electrophoresis; S5, result analysis: According to the analysis of the migration trend of the same protein in the cell samples of the experimental group and the corresponding blank control group, the identity of the human cell protein is judged Degree of deamidation modification. The method has the advantages of simple operation and strong specificity, can shorten the experimental period for identifying protein deamidation reaction, save experimental cost, and can be applied to proteomic analysis related to human tumor cells. |
| priorityDate | 2022-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.