http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115235969-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2fb7cb86a8b99fcfeb3aa3d19a51c8de |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0679 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-08 |
| filingDate | 2022-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0cbfaa88c88600e2bc170f2d95f9ff6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ca4368fe75f47253bc4e0d9bfad4c59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fde680a507d38ad2cc9986687e7d2ede |
| publicationDate | 2022-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115235969-A |
| titleOfInvention | A new method for evaluating the small intestinal permeability of drugs |
| abstract | The present invention proposes a new method for evaluating the permeability of the small intestine of drugs, using small intestinal organoid cells and Caco-2 cells in The device was co-cultured to form a dense cell layer to mimic the intestinal wall of the small intestine. The drug intestinal wall permeability test is completed in the device and the apparent permeability coefficient, efflux ratio and recovery rate of the drug to be tested are calculated; small intestinal organoid cells are obtained by reprogramming and directed differentiation of PBMC cells. In the present invention, by mixing the differentiated small intestinal organoid cells with the commonly used colon cancer cell Caco-2 in different proportions, it is found that the mixed cells can not only form a complete and dense cell layer faster, but also the resistance can be increased about 7 days after plating Achieving ≥500Ω/cm 2 and the resulting dense cell layer, as well as the Caco‑2 cell layer after 20 days of culture, can be effectively used to assess the intestinal permeability of compounds in vitro. |
| priorityDate | 2022-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.