| abstract |
The patent of the present invention discloses a sequencing method for targeting and enriching bacterial 16S rRNA gene based on nanopore sequencing. Cut the conserved sequence of the 16S rRNA genome of the pathogenic bacterial microorganism, selectively add the A sticky end tail at the enzyme cutting site, connect the sequencing adapter, and compare the sequence with the gene database according to the sequencing data to obtain the strain of the bacteria to be identified; Patent of the present invention The V2-V3 conserved gene of stable 16S rRNA is used for targeted enrichment, and V2-V3 is used as the sequencing starting point, and the gene coverage at both ends of the 16S rRNA obtained by detection is higher, the detection range is wider, and the classification result is more credible, the present invention The specific targeting of bacterial 16S rRNA gene library construction can effectively avoid the interference of the host genome, reduce the proportion of human origin of the background host, improve the detection sensitivity and detection efficiency of pathogens, shorten the detection time, and improve the detection efficiency of nanopores. |