| abstract |
The invention discloses a chicken anti-Eimeria maxima oral recombinant spore vaccine and a preparation method thereof, and relates to the field of agricultural science and technology. Repair the intestinal flora imbalance caused by coccidial infection, resulting in the problem of low breeding efficiency. It includes the following steps: Step 1: Prepare the preparation materials and equipment, perform quality inspection in turn, and check if it can be used normally, and five mediums need to be prepared; Step 2: Carry out the construction of the recombinant plasmid pDGIEF‑EMIMP1C; Step 3: Carry out Construction of the recombinant Bacillus subtilis; Step 4: carry out the identification of the recombinant Bacillus subtilis, wherein the amylase activity needs to be detected first, then the expression product is analyzed by SDS-PAGE, and then the expression product is identified by western-blot; wherein, the step The medium in one comprises a medium body, and the upper end of the medium body is provided with a medium cover. |