| abstract |
The invention relates to a preparation method of an electrochemiluminescence immunosensor based on cuprous oxide nano-cube quenched iron-doped graphite carbon nitride. In the present invention, Fe -Nx groups coordinate to the nitrogen tank of g-C 3 N 4 by coordinating with the N atoms of g-C 3 N 4 to form key active sites, and iron-doped graphitic carbon nitride The strong and continuous electrochemiluminescence signal required by the sensor is provided. The electrochemiluminescence emission spectrum of iron-doped graphitic carbon nitride and the ultraviolet absorption spectrum of cuprous oxide have a large overlap, and electrochemiluminescence-resonance energy transfer can occur between the two. ECL of iron-doped graphitic carbon nitride The emission can be efficiently quenched by cuprous oxide. Using iron-doped graphitic carbon nitride to bind to the primary antibody of the soluble fragment of cytokeratin 19 to form a primary antibody marker, and cuprous oxide to bind to the secondary antibody of the soluble fragment of cytokeratin 19 to form a secondary antibody marker, simplifying the sensor During the construction process, ultrasensitive detection of soluble fragments of cytokeratin 19 was achieved with a detection limit of 15.53 fg/mL. |