http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115216491-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d6caadbc395b3d6ec4ecb6783f1a50d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8212 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate | 2022-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5a2bbe896661ade42902e78e7f2eb72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_930fd69c8f06d68d91cfba7e3f3383d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6dd564abf17ed0623a1183babfccf93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_756d17cb8ea2a66fa6f6798d19bccfc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f35295dcf935da8ea64bcba7af229ada http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99833e278f73bcbb68624273afbbf465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c502a604b189b437af4904a344e58a6 |
| publicationDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115216491-A |
| titleOfInvention | A kind of method for efficient genetic transformation of bitter gourd |
| abstract | The invention discloses a method for high-efficiency genetic transformation of bitter gourd, which comprises the following steps: immersing pre-cultured explants into a transfection solution, ultrasonically treating them with 40-50 KHz for 20-30 minutes, and then transferring the explants and the transfection solution into a non-toxic solution together. Vacuum treatment was carried out in the bacterial syringe; the vacuumed explants were sequentially transferred to co-culture medium for co-culture, and resistance screening culture was carried out on the screening medium; The tissue was excised and transferred to rooting medium for rooting culture. The present invention enhances the regeneration ability of bitter gourd explants by using the soon-to-mature seeds with strong differentiation ability as the transformation receptors, the cotyledon nodes as the explants, and adding the growth regulator GFR4 to the transformation vector, thereby greatly increasing the It improves the regeneration rate and breaks through the bottleneck of difficult regeneration of bitter gourd. In addition, the infection was carried out by ultrasonic treatment and vacuuming, which greatly improved the transformation efficiency and laid a foundation for the realization of gene genetic transformation. |
| priorityDate | 2022-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.