| abstract |
The invention discloses a method for culturing deodorizing lactic acid bacteria, which is characterized in that sample collection: a total of about 30 g of samples are collected from the middle and bottom layers of the accumulated pig manure, put into a sterile ziplock bag and then stored in an ice box, and delivered within 3 hours. To the laboratory, it is used for the isolation and screening of target strains. The isolation and screening of strains: take 5g of the collected samples into a 250 mL conical flask filled with 100 mL of sterile water, put them in a shaker, and place them in a shaker at a temperature of 30 °C and a rotating speed. After being treated for 2 h at 200 r/min, it was taken out and kept at rest for 1 h. Take 5 mL of the supernatant into a 250 mL conical flask containing 50 mL of MRS medium, put it in a shaker, and treat it in a shaker with a temperature of 30 °C and a rotation speed of 120 r/min for 48 hours, and enrich the processed samples. 10-fold dilution method, draw 0.1 mL from 10-3 , 10-5 , 10-7 , 10-9 dilution and coat on the MRS solid plate, and cultivate at a constant temperature of 30°C; the culture method of this deodorizing lactic acid bacteria promotes It has the advantages of producing and promoting growth, producing acid, inhibiting the growth of spoilage microorganisms, and effectively removing malodorous gases. |