| abstract |
The invention discloses a multi-layer cascaded neural network microfluidic chip and a preparation method thereof, and relates to a sensor manufacturing technology. The chip system is composed of a microelectrode array chip used for electrophysiological detection and regulation and a microfluidic chip used for zonal cultivation of neuron networks. The microelectrode array chip includes microelectrodes, counter electrodes and other components; the microfluidic chip includes multiple A cell culture tank, multiple groups of microchannels connecting the culture tanks, and synaptic control chambers. The chip of the invention is integrated, and has the function of cultivating different neuron networks in different regions and the ability to detect and regulate neuron networks in time and space. The connection relationship between the networks; the synaptic control room can achieve precise regulation of synapses; the microelectrodes at the bottom can be used to detect changes in the electrical activity of the neuron network after stimulation and regulation. |