| abstract |
The invention discloses a microorganism purification device for mass spectrometry identification of blood microorganism species and a method for using the same, comprising a general controller, a coarse filter, a cracker, a fine filter, a waste liquid bottle and a collection bottle, and is characterized in that: the crude The lower end of the filter is communicated with the upper end of the cracker, the lower end of the cracker is communicated with the upper end of the fine filter, the lower end of the fine filter is communicated with the upper end of the waste liquid bottle, and the side wall of the fine filter is communicated with the collection bottle; The coarse filter membrane and the fine filter membrane are installed inside the lower section of the fine filter, and the upper end of the fine filter is connected to the micro water pump. The invention reduces manual intervention and improves the experimental accuracy by electronically controlling the experimental steps of the main controller; firstly, large volumes of substances in the sample are filtered out to improve the lysis efficiency; residual blood cells are lysed by the blood cell lysate, and then filtered and cleaned at the fine filter membrane It is discharged and collected, which simplifies the experimental steps, does not require high level of laboratory personnel, and has high purification rate and high identification accuracy. |