| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 |
| filingDate |
2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4b2f2d1bf48c2acbcd9fea0bd0a9d5e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07f081918c8f4812c3a3c150d7698857
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36865c8528557a175d414694db8acdfb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca9cb0f84193bcdd70df4e4f5ad52036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1de60c31030b83f278218215510a69c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3d3106b4a7753e0572418c745ee8e8f |
| publicationDate |
2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-115215941-A |
| titleOfInvention |
Nanoscale dual-enzyme cascade reactor based on covalent self-assembly of spontaneous isopeptide bonds |
| abstract |
The invention provides a nanometer double-enzyme cascade reactor based on spontaneous isopeptide bond covalent self-assembly, which is composed of SpyCatcher-ATA117 fusion expression transaminase and SpyTag-ADH fusion expression dehydrogenase self-assembly. In the present invention, SpyTag and SpyCatcher are respectively fused with yeast alcohol dehydrogenase ADH and transaminase ATA117, and the frameless self-assembly of SpyCatcher-ATA117 and SpyTag-ADH is realized through the specific interaction between SpyCatcher and SpyTag. The multi-enzyme cascade reactor catalyzed the cascade reaction and significantly improved the amination efficiency of the substrate BPO, thereby establishing a process for the preparation of R‑BPA by amination of BPO with high efficiency. This process control strategy provides a reference for later industrial applications. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113621031-A |
| priorityDate |
2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |